Oxidative Phosphorylation in Fractionated Bacterial Systems
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چکیده
منابع مشابه
Oxidative phosphorylation in fractionated bacterial systems. XXV. Studies on the involvement of metal in Mycobacterium phlei.
The involvement of metal in oxidation and coupled phosphorylation by cell-free preparations of Mycobacterium phlei was examined. The oxidation of succinate was inhibited by metal-chelating agents, but with /3-hydroxybutyrate, chelating agents exhibited an uncoupling effect. The site of inhibition was found to be between the flavoprotein and the cytochrome b regions of the electron transport cha...
متن کاملOxidative Phosphorylation in Fractionated Bacterial Systems. Xiii. Effects of Photooxidation on the Soluble Coupling Factors.
* This investigation was supported in part by a grant from the National Institutes of Health, U.S. Public Health Service. t Contribution no. 3056. I Platt, A., and C. Niemann, these PROCEEDINGS, 50, 817 (1963). 2 Peterson, R. L., K. W. Hubele, and C. Niemann, Biochemistry, 2, 942 (1963). 3Hein, G. E., and C. Niemann, J. An. Chem. Soc., 84, 4487, 4495 (1962). 4Applewhite, T. H., R. B. Martin, an...
متن کاملOxidative Phosphorylation in Fractionated Bacterial Systems XVIII. PHOSPHORYLATION COUPLED TO DIFFERENT SEGMENTS OF THE RESPIRATORY CHAINS OF MYCOBACTERIUM PHLEI*
A number of bacterial systems have been described which will carry out oxidative phosphorylat,ion (1-12). In general these systems differ from intact mammalian mitochondrial systems in that the bacterial systems have lower P:O ratios and fail to exhibit respiratory control. Loss of sites of phosphorylation (13, 14) and nonphosphorylative electron transport bypass reactions (13, 15-18) has been ...
متن کاملOxidative phosphorylation in fractionated bacterial systems. 18. Phosphorylation coupled to different segments of the respiratory chains of Mycobacterium phlei.
A number of bacterial systems have been described which will carry out oxidative phosphorylat,ion (1-12). In general these systems differ from intact mammalian mitochondrial systems in that the bacterial systems have lower P:O ratios and fail to exhibit respiratory control. Loss of sites of phosphorylation (13, 14) and nonphosphorylative electron transport bypass reactions (13, 15-18) has been ...
متن کاملOxidative phosphorylation in fractionated bacterial systems. XXIX. The involvement of nonheme iron in the respiratory pathways of Mycobacterium phlei.
Spectrophotometric evidence for the reduction of nonheme iron in the respiratory particles of Mycobacferium phlei was obtained by trapping the metal as ferrous o-phenanthrolinate. The protein-bound iron-o-phenanthroline complex had an absorption maximum at 510 mp, and the extent of reduction of nonheme iron was followed by observing the increase in absorbance at 510 to 540 rnp on the addition o...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69616-4